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Elisa has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries It is a method of quantifying an antigen immobilized on a solid surface. In the most simple form of an elisa, antigens from the sample to be tested are attached to a surface.

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Learn about the different methods for performing an elisa assay for protein quantitation, including assay design strategies and reagents. Laboratory scientists use this technique for several medical tests — from diagnosing infections to confirming pregnancy. Elisa is a laboratory technique that detects certain antibodies, antigens and other substances in your blood, pee or other bodily fluid